Immune response induced by conjunctival immunization with polymeric antigen BLSOmp31 using a thermoresponsive and mucoadhesive in situ gel as vaccine delivery system for prevention of ovine brucellosis
- Díaz, Alejandra Graciela
- Quinteros, Daniela Alejandra
- Gutiérrez, Silvina Elena
- Rivero, Mariana Alejandra
- Palma, Santiago Daniel
- Allemandi, Daniel Alberto
- Pardo, Romina Paola
- Zylberman, Vanesa
- Goldbaum, Fernando Alberto
- Estein, Silvia Marcela
DOIAbstract
Control of ovine brucellosis with subcellular vaccines can solve some drawbacks associated with the use of Brucella melitensis Rev.1. Previous studies have demonstrated that the polymeric antigen BLSOmp31 administered by parenteral route was immunogenic and conferred significant protection against B. ovis in rams. Immunization with BLSOmp31 by conjunctival route could be efficient for the induction of mucosal and systemic immune responses. In this work, we evaluated the conjunctival immunization using a thermoresponsive and mucoadhesive in situ gel composed of Poloxamer 407 (P407) and chitosan (Ch) as vaccine delivery system for BLSOmp31 in rams. Serum samples, saliva, lacrimal, preputial and nasal secretions were analyzed to measure specif...
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