EU Member States are mandated to reach a minimum of 10% renewable energy consumed in transport by 2020. To comply with this goal, biofuels must meet the Renewable Energy Directive (RED) sustainability requirements. Further policy developments at the European level are expected to lead to the progressive substitution of first-generation (food crop-based) with second- (non-edible crops-based) and third-generation (algae and microalgae-based) biofuels. This paper explores the economic feasibility and environmental sustainability of producing second-generation biofuel in Italy from Brassica Carinata, a non-edible flowering plant of the Brassicaceae family, in a context of rotation with wheat (and eventually other crops). The framework conside...