Additional file 8: of Bidirectional promoters exhibit characteristic chromatin modification signature associated with transcription elongation in both sense and antisense directions

Figure S8. H3K36me3 distribution on bidirectional gene with different intergenic region in H1 ES cells. The figure shows enrichment of H3K79me1 at the bidirectional genes pairs with intergenic distance up to 1000 bp. Intergenic distance is represented as the number of nucleosomes that could potentially be accommodated. Data are shown for the gene pairs which have intergenic region that could contain 2 to 6 nucleosomes assuming 170 bp length for wrapping around each octamer and inclusive of 20 bp linker. Cumulative expression is calculated by summation of fold enrichment signal at every location in a 4 KB window for each category and dividing by the highest value of signal in the respective category as described in ‘Methods’. (A) Cumulative enrichment of H3K36me3 on bidirectional genes which are asymmetric with respect to their expression profiles. (B) Cumulative enrichment of H3K36me3 on bidirectional genes which are symmetric with respect to their expression profiles. (PDF 829 kb