Additional file 1: of Bidirectional promoters exhibit characteristic chromatin modification signature associated with transcription elongation in both sense and antisense directions

Figure S1. Design of dual reporter vector and bidirectional transcription from bidirectional promoters cloned in antisense orientation. (A) The strategy for introducing eGFP and mCherry under a common regulatory DNA element is shown. This vector construct is designed to provide a quantitative readout in live cells based on strand-specific promoter activity. Intense red and green colors indicate direction of promoter activity. Three constructs are shown in the scheme, in middle is pDR vector with no promoter element, Right side shows construct with CMV promoter antisense to mCherry, left side shows construct with CMV promoter antisense to eGFP. (B) Clones from Fig. 1 in their antisense orientation show the same outcome. (PDF 1427Â kb