Additional file 9: of A genome-wide assessment of conserved SNP alleles reveals a panel of regulatory SNPs relevant to the peripheral nerve

Figure S6. CRISPR cuts all alleles in S16 cells. A wild-type specific diagnostic PCR was performed on rSOX-4 Clone 1-B and 2-B, and PCR products were sequenced. (A) A single base pair insertion (yellow box) of an adenine was identified in the remaining non-recombined alleles of rSOX-4 Clone 1-B that resides within the gRNA-1 recognition site. (B) A 79 base-pair deletion was detected encompassing the gRNA-2 cut site in rSOX-4 Clone 2-B. The yellow box represents the expected 5′ sequence, and the blue box represents the 3′ sequence. In both panels, the rn5 genome sequence is the expected sequence, and gRNA-1, SOX10 consensus site, and gRNA-2 are labeled and indicated by lines under the nucleotide sequences. (AI 1471 kb