Sinefungin suppressed expression of extracellular matrix (ECM)-associated genes and H3K4me1 level at <i>Col1A2</i> promoters.

<p>Quantitative real-time polymerase chain reaction (PCR) analysis of mRNA expression of (A) <i>ACTA2</i> (<i>α-SMA</i>), (B) <i>Col1A2</i>, (C) <i>CTGF</i> and (D) <i>PAI-1</i> in HPMCs (standardized to glyceraldehyde 3-phosphate dehydrogenase [<i>GAPDH</i>]). (E) Representative chromatin immunoprecipitation (ChIP) assay of the binding of the H3K4me1 protein (H3K4me1-Ab) to <i>Col1A2</i> promoters in HPMCs. Negative control: mouse immunoglobulin G (IgG). Full-length gels are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0196844#pone.0196844.s005" target="_blank">S5 Fig</a>. Data are means ± S.D. *, <i>P</i> < 0.05 (one-way ANOVA followed by the <i>post hoc</i> test using <i>t</i> test with Bonferroni correction; <i>n</i> = 5 samples per group).