Sinefungin inhibited TGF-β1-induced fibrotic markers and H3K4me1 in HPMCs.

<p>Representative Western blotting results for the expression of (A) α-SMA (B) secreted fibronectin and (C) zonula occludens-1 (ZO-1) of HPMCs. α-tubulin was used as an internal control. Lower panel: quantification. (D) Representative Western blotting analysis showing level of H3K4me1 in HPMCs stimulated by TGF-β1. H3 was used as the internal control. Lower panel: quantification. Full-length blots are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0196844#pone.0196844.s004" target="_blank">S4 Fig</a>. Data are means ± S.D. *, <i>P</i> < 0.05 (one-way ANOVA followed by the <i>post hoc</i> test using <i>t</i> test with Bonferroni correction; <i>n</i> = 5 samples per group).