Additional file 2: Standard protocol of a RecET-assisted CRISPR-Cas9 genome editing in Corynebacterium glutamicum
Additional file 1: Figure S1. Graphical depiction of the positions of the genomic target sites of Ga...
Abstract Background Corynebacterium glutamicum is an important industrial strain for the production ...
Not AvailableThis second volume provides new and updated methods detailing advancements in CRISPR-Ca...
Additional file 1: Figure S1. Colony PCR verification of cas9 integration. Figure S2. Colony PCR ver...
Abstract Background Extensive modification of genome is an efficient manner to regulate the metaboli...
Additional file 1: Figure S1. PCR verification of ldhA deletion using CRISPR/Cas9 and the two-step e...
Additional file 8. Flow-chart of 3 rounds of continual genome editing in P. putida KT2440
Abstract Background Corynebacterium glutamicum is an important industrial workhorse and advanced gen...
Additional file 2: Figure S2. Verification of cre-1 gene deletions in selected transformants with co...
Additional file 2: Methods. Methods for growth and phenotypic analysis of fission yeast, molecular b...
Additional file 2. Profile and sequence of rfp editing plasmid pBBR1-Cas9-rfpF-rfpR
The development of new genetic tools plays a key role in the establishment of new and/or improved mi...
Additional file 6: Figure S6. Determination of PtrpC-neo cassette copy numbers in the disrupted muta...
Cleto S, Vold Korgaard Jensen J, Wendisch VF, Lu TK. Corynebacterium glutamicum metabolic engineerin...
Genome engineering of Corynebacterium glutamicum, an important industrial microorganism for amino ac...
Additional file 1: Figure S1. Graphical depiction of the positions of the genomic target sites of Ga...
Abstract Background Corynebacterium glutamicum is an important industrial strain for the production ...
Not AvailableThis second volume provides new and updated methods detailing advancements in CRISPR-Ca...
Additional file 1: Figure S1. Colony PCR verification of cas9 integration. Figure S2. Colony PCR ver...
Abstract Background Extensive modification of genome is an efficient manner to regulate the metaboli...
Additional file 1: Figure S1. PCR verification of ldhA deletion using CRISPR/Cas9 and the two-step e...
Additional file 8. Flow-chart of 3 rounds of continual genome editing in P. putida KT2440
Abstract Background Corynebacterium glutamicum is an important industrial workhorse and advanced gen...
Additional file 2: Figure S2. Verification of cre-1 gene deletions in selected transformants with co...
Additional file 2: Methods. Methods for growth and phenotypic analysis of fission yeast, molecular b...
Additional file 2. Profile and sequence of rfp editing plasmid pBBR1-Cas9-rfpF-rfpR
The development of new genetic tools plays a key role in the establishment of new and/or improved mi...
Additional file 6: Figure S6. Determination of PtrpC-neo cassette copy numbers in the disrupted muta...
Cleto S, Vold Korgaard Jensen J, Wendisch VF, Lu TK. Corynebacterium glutamicum metabolic engineerin...
Genome engineering of Corynebacterium glutamicum, an important industrial microorganism for amino ac...
Additional file 1: Figure S1. Graphical depiction of the positions of the genomic target sites of Ga...
Abstract Background Corynebacterium glutamicum is an important industrial strain for the production ...
Not AvailableThis second volume provides new and updated methods detailing advancements in CRISPR-Ca...