Additional file 8. Flow-chart of 3 rounds of continual genome editing in P. putida KT2440
Additional file 1: Figure S1. Colony PCR verification of cas9 integration. Figure S2. Colony PCR ver...
Additional file 2: Figure S2. Verification of cre-1 gene deletions in selected transformants with co...
Pseudomonas putida KT2440 is the only fully sequenced P. putida strain. Thus, for transcriptomics an...
Abstract Background The soil bacterium Pseudomonas putida KT2440 is a “generally recognized as safe”...
Additional file 2: Standard protocol of a RecET-assisted CRISPR-Cas9 genome editing in Corynebacteri...
Additional file 6: Figure S6. Determination of PtrpC-neo cassette copy numbers in the disrupted muta...
Additional file 2. Profile and sequence of rfp editing plasmid pBBR1-Cas9-rfpF-rfpR
Summary: Pseudomonas species are a large class of gram-negative bacteria that exhibit significant bi...
© 2019 The Authors.Implementation of single‐stranded DNA (ssDNA) recombineering in Pseudomonas putid...
Additional file 2: Methods. Methods for growth and phenotypic analysis of fission yeast, molecular b...
Additional file 1: Figure S1. rfp editing identified by agarose gel electrophoresis and sequencing. ...
Horizontal gene transfer (HGT) is the exchange of genetic material between bacterial cells. Megaplas...
Pseudomonas putida KT2440 is the only fully sequenced P. putida strain. Thus, for transcriptomics an...
The investigation of gene function often involves gene knock-out or mutational analysis. These proce...
Sequencing data for "RNA editing, RNA modifications, and transcriptional units in Listeria monocytog...
Additional file 1: Figure S1. Colony PCR verification of cas9 integration. Figure S2. Colony PCR ver...
Additional file 2: Figure S2. Verification of cre-1 gene deletions in selected transformants with co...
Pseudomonas putida KT2440 is the only fully sequenced P. putida strain. Thus, for transcriptomics an...
Abstract Background The soil bacterium Pseudomonas putida KT2440 is a “generally recognized as safe”...
Additional file 2: Standard protocol of a RecET-assisted CRISPR-Cas9 genome editing in Corynebacteri...
Additional file 6: Figure S6. Determination of PtrpC-neo cassette copy numbers in the disrupted muta...
Additional file 2. Profile and sequence of rfp editing plasmid pBBR1-Cas9-rfpF-rfpR
Summary: Pseudomonas species are a large class of gram-negative bacteria that exhibit significant bi...
© 2019 The Authors.Implementation of single‐stranded DNA (ssDNA) recombineering in Pseudomonas putid...
Additional file 2: Methods. Methods for growth and phenotypic analysis of fission yeast, molecular b...
Additional file 1: Figure S1. rfp editing identified by agarose gel electrophoresis and sequencing. ...
Horizontal gene transfer (HGT) is the exchange of genetic material between bacterial cells. Megaplas...
Pseudomonas putida KT2440 is the only fully sequenced P. putida strain. Thus, for transcriptomics an...
The investigation of gene function often involves gene knock-out or mutational analysis. These proce...
Sequencing data for "RNA editing, RNA modifications, and transcriptional units in Listeria monocytog...
Additional file 1: Figure S1. Colony PCR verification of cas9 integration. Figure S2. Colony PCR ver...
Additional file 2: Figure S2. Verification of cre-1 gene deletions in selected transformants with co...
Pseudomonas putida KT2440 is the only fully sequenced P. putida strain. Thus, for transcriptomics an...
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