Propofol upregulate HO-1 expression and nuclear localization of Nrf2 under oxidative stress conditions.

<p><b>A</b>: mRNA expression profile for HO-1. n = 3. The data are expressed as mean ± SEM. <b>B</b>: Western blot analysis of HO-1 in H9c2 cells. <b>C</b>: Quantification of the density of expression level of HO-1. n = 3. The data are expressed as mean ± SEM. <b>D</b>: Western blot analysis of Nrf2 in H9c2 cells. <b>E</b>: Quantification of the density of expression level of total Nrf2. n = 3. The data are expressed as mean ± SEM. <b>F</b>: Western blot analysis of Nrf2 in total lysates, nuclear lysates and cytoplasmic lysates of H9c2 cells. <b>G</b>: Western blot analysis of Nrf2 in nuclear lysates and cytoplasmic lysates of H9c2 cells. <b>H</b>, <b>I</b>: Quantification of the density of expression level of nuclear Nrf2 and cytoplasmic Nrf2. <b>J</b>: Representative immunocytochemical labeling of Nrf2 in H9c2 cells treated with H₂O₂ and propofol (PR). Scale bar, 50 μm. Control (con) means no treatment. *<i>P</i> < 0.05, **<i>P</i> < 0.01.