ELISA-EC50 analysis of A244.AE and 6240.B gp120 binding to mAbs recognizing critical epitopes.
- Yingxia Wen (2455015)
- Hung V. Trinh (5146997)
- Christine E. Linton (5147003)
- Chiara Tani (3572036)
- Nathalie Norais (159651)
- DeeAnn Martinez-Guzman (5147021)
- Priyanka Ramesh (1374711)
- Yide Sun (334156)
- Frank Situ (5147006)
- Selen Karaca-Griffin (5147015)
- Christopher Hamlin (5147024)
- Sayali Onkar (5147009)
- Sai Tian (1795663)
- Susan Hilt (3149133)
- Padma Malyala (5147012)
- Rushit Lodaya (5147000)
- Ning Li (45258)
- Gillis Otten (5147018)
- Giuseppe Palladino (211627)
- Kristian Friedrich (816041)
- Yukti Aggarwal (816042)
- Celia LaBranche (170898)
- Ryan Duffy (779151)
- Xiaoying Shen (164029)
- Georgia D. Tomaras (164058)
- David C. Montefiori (108747)
- William Fulp (459224)
- Raphael Gottardo (1558)
- Brian Burke (248053)
- Jeffrey B. Ulmer (120136)
- Susan Zolla-Pazner (156004)
- Hua-Xin Liao (161409)
- Barton F. Haynes (140266)
- Nelson L. Michael (132309)
- Jerome H. Kim (132312)
- Mangala Rao (147350)
- Robert J. O’Connell (519953)
- Andrea Carfi (203340)
- Susan W. Barnett (120170)
Publication date
April 2018
DOIAbstract
<p>ELISA-EC50 values for the binding of the gp120s to a panel of well-characterized mAbs were determined as described in Methods. All results shown here were confirmed by Surface Plasmon Resonance (SPR; some confirmatory analyses and additional data were shown for an expanded panel of mAbs in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0194266#pone.0194266.s004" target="_blank">S4 Fig</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0194266#pone.0194266.s005" target="_blank">S5 Fig</a>. EC50s of mAb binding to A244.AE gp120 (circles) and binding to 6240.B gp120 (triangles) were shown. Each symbol (circle/triangle) represents a replicate measurement.</p
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