Fine specificity of mAbs elicited by sequential H1N1 exposure.

<p>(A, B) mAbs created from PR8 pre-exposed mice that were boosted either with PR8 (A) or S12a (B) were tested for binding to a panel of viruses by ELISA. We first completed a dose titration of each mAb on ELISA plates coated with PR8. We determined the lowest concentration of each mAb at which PR8 ELISA signal was within 90% of Bmax, and we tested this mAb concentration against all of the mutant viruses. The shade of color in each square is relative to the percentage of binding to that virus compared to PR8 binding with the darkest color being 100% and white being 0%. The coloring scheme (Sa = green, Sb = purple, Ca = blue, Cb = red) matches the coloring scheme of the HA structure shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005806#ppat.1005806.g001" target="_blank">Fig 1</a>. mAbs are IgG unless otherwise noted.