目的分析柯萨奇B3病毒(CVB3)中国分离株编码主要中和抗原的VP1基因序列.方法用逆转录PCR技术扩增CVB3中国分离株的VP1基因,通过TA克隆法构建pCR2.1-VP1并进行核苷酸序列的测定分析.结果测序发现CVB3中国分离株与CVB3 Nancy株VP1基因均编码293个氨基酸,二者存在59个核苷酸差异, 其中10处影响到氨基酸的编码,其余均为同义变异.结论 CVB3中国分离株与Nancy株VP1基因序列存在一定的差异,该差异对免疫学性状的影响有待进一步鉴定.国家自然科学基金; 黑龙江省自然科学基金中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)018-102
Nineteen CVA9 isolates were obtained between 2010 and 2019 from six provinces of mainland China, usi...
目的对9个TTV新分离株全基因序列测定、基因结构及基因分型的研究.方法从9名TTV第4基因群感染阳性的婴儿血清中抽提其DNA,用longinverted PCR扩增出全基因组、克隆和测定全基因组序列,...
目的构建恶性疟原虫海南株(FCC1/HN)CSP3′末端基因的原核表达载体,并对CSP3′末端基因进行序列测定.方法根据恶性疟原虫CSP编码的基因序列设计引物,采用PCR技术扩增出CSP3′末端基因,...
中国近年的手足口病(HFMD)流行中呈柯萨奇病毒16(CA16)和肠道病毒EV71交替或共同流行的趋势,目前GenBank数据库中CA16全基因组序列较少,本研究对北京地区2008年的1株CA16全基...
测定和分析霍乱弧菌分型噬菌体VP3基因组序列,并为El Tor型霍乱弧菌两类菌株的分型方法原理提供研究基础.鸟枪法构建VP3噬菌体全基因组随机文库;测序拼接成最小重叠群,引物步移法填补缝隙序列,拼接后...
A human coxsackievirus B3 (CVB3), designated strain Beijing0811, was isolated from a child diagnosed...
A human coxsackievirus B3 (CVB3), designated strain Beijing0811, was isolated from a child diagnosed...
目的:探讨逆转录聚合酶链反应(RT-PCR)方法在检测柯萨奇病毒B_3(CoxB_3)感染中的作用.方法:采用RT-PCR方法对北京某医院3l对(62例)疑似有母婴传播性CoxB_3 感染的新生儿和母...
Coxsackievirus A4 (CVA4) is one of the most prevalent pathogens associated with hand, foot and mouth...
目的以构建的柯萨奇病毒B组3型(CVB3)VP1基因的真核表达系统pCEP4-CVB3VP1作为基因疫苗,评价其对CVB3实验性心肌炎的预防作用.方法大量制备pCEP4-CVB3VP1,提纯后将其接种...
Coxsackievirus B3 (CVB3) is a pathogenic Enterovirus of the picornavirus family, with a single-stran...
Coxsackievirus A4 (CVA4) is one of the most prevalent pathogens associated with hand, foot and mouth...
Background/PurposeIn recent years, coxsackievirus B3 (CV-B3) has been determined as a dominant enter...
Coxsackievirus A4 (CVA4) is one of the most prevalent pathogens associated with hand, foot and mouth...
Coxsackievirus A4 (CVA4) is one of the most prevalent pathogens associated with hand, foot and mouth...
Nineteen CVA9 isolates were obtained between 2010 and 2019 from six provinces of mainland China, usi...
目的对9个TTV新分离株全基因序列测定、基因结构及基因分型的研究.方法从9名TTV第4基因群感染阳性的婴儿血清中抽提其DNA,用longinverted PCR扩增出全基因组、克隆和测定全基因组序列,...
目的构建恶性疟原虫海南株(FCC1/HN)CSP3′末端基因的原核表达载体,并对CSP3′末端基因进行序列测定.方法根据恶性疟原虫CSP编码的基因序列设计引物,采用PCR技术扩增出CSP3′末端基因,...
中国近年的手足口病(HFMD)流行中呈柯萨奇病毒16(CA16)和肠道病毒EV71交替或共同流行的趋势,目前GenBank数据库中CA16全基因组序列较少,本研究对北京地区2008年的1株CA16全基...
测定和分析霍乱弧菌分型噬菌体VP3基因组序列,并为El Tor型霍乱弧菌两类菌株的分型方法原理提供研究基础.鸟枪法构建VP3噬菌体全基因组随机文库;测序拼接成最小重叠群,引物步移法填补缝隙序列,拼接后...
A human coxsackievirus B3 (CVB3), designated strain Beijing0811, was isolated from a child diagnosed...
A human coxsackievirus B3 (CVB3), designated strain Beijing0811, was isolated from a child diagnosed...
目的:探讨逆转录聚合酶链反应(RT-PCR)方法在检测柯萨奇病毒B_3(CoxB_3)感染中的作用.方法:采用RT-PCR方法对北京某医院3l对(62例)疑似有母婴传播性CoxB_3 感染的新生儿和母...
Coxsackievirus A4 (CVA4) is one of the most prevalent pathogens associated with hand, foot and mouth...
目的以构建的柯萨奇病毒B组3型(CVB3)VP1基因的真核表达系统pCEP4-CVB3VP1作为基因疫苗,评价其对CVB3实验性心肌炎的预防作用.方法大量制备pCEP4-CVB3VP1,提纯后将其接种...
Coxsackievirus B3 (CVB3) is a pathogenic Enterovirus of the picornavirus family, with a single-stran...
Coxsackievirus A4 (CVA4) is one of the most prevalent pathogens associated with hand, foot and mouth...
Background/PurposeIn recent years, coxsackievirus B3 (CV-B3) has been determined as a dominant enter...
Coxsackievirus A4 (CVA4) is one of the most prevalent pathogens associated with hand, foot and mouth...
Coxsackievirus A4 (CVA4) is one of the most prevalent pathogens associated with hand, foot and mouth...
Nineteen CVA9 isolates were obtained between 2010 and 2019 from six provinces of mainland China, usi...
目的对9个TTV新分离株全基因序列测定、基因结构及基因分型的研究.方法从9名TTV第4基因群感染阳性的婴儿血清中抽提其DNA,用longinverted PCR扩增出全基因组、克隆和测定全基因组序列,...
目的构建恶性疟原虫海南株(FCC1/HN)CSP3′末端基因的原核表达载体,并对CSP3′末端基因进行序列测定.方法根据恶性疟原虫CSP编码的基因序列设计引物,采用PCR技术扩增出CSP3′末端基因,...
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