从枯草芽孢杆菌Bacillus subtilis中分离克隆果聚糖合酶(1evansucrase)基因sacB,构建置于CaMV35s启动子调控下的表达载体,通过农杆菌介导,将sacB基因导入番茄栽培品种碧娇.Southe rn杂交证明,目的基因已经整合到番茄基因组中.RT-PCR分析表明,sacB基因在转基因植株中获得转录.在含50 mg·ml-1卡那霉素生根培养基中转化植株生根良好.抗冻试验、叶片电导率测定、多糖含量测定的试验结果均表明,果聚糖合酶基因赋予转基因番茄良好的抗寒性状.国家自然科学基金中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)081193-11973
The successful transformation of callus derived protoplasts of the tomato genotype MSK93 was recent...
Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (Fol) is one of the most devastating d...
Genetic engineering has been used as an alternative method to classical breeding procedures to enhan...
通过土壤农杆菌(Agrobacterium tumefaciens)的介导,将烟草花叶病毒(TMV)的54103蛋白cDNA转入番茄植物中,得到了转基因植株.这些植株抗卡那霉素,具有胭脂碱合成酶的活性...
[目的]研究转GO基因番茄的抗病机制.[方法]通过测定转GO基因番茄和对照未转基因番茄接种叶霉菌Fulvia fulva后,体内超氧物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、多酚...
将可以诱发植物对病原菌抗性的甜椒hrap基因置于35S启动子的驱动下,经农杆菌介导引入番茄.再生植株经过含卡那霉素的培养基的选择,后经PCR检测和Southern杂交鉴定含有目标基因.Northern...
对转基因抗黄瓜花叶病毒(CMV)蕃茄进行了安全性评价.结果:CMV蕃茄急性毒性半数致死量LD50>10 g/kg,属实际无急性毒性物质.经微核试验、精子畸变试验及Ames试验均未发现有致突变作用...
本研究室先前的數量性狀基因座遺傳定位研究指出，野生番茄L3708 (Solanum pimpinellifolium L.) 除了在第九對染色體具有晚疫病抗病基因Ph-3以外，於第二對染色體上亦具有另...
The pto gene, responsible for resistance to Pseudomonas syringae pv. tomato, was transferred to toma...
Développer des plantes résistantes aux pathogènes nécessite d’identifier des sources de résistance, ...
从枯草芽孢杆菌(Bacillussubtilis)抗脯氨酸结构类似物突变株中克隆得到脯氨酸合成途径中的关键酶基因proB(编码γ-谷氨酰胺激酶)和proA(编码谷氨酰胺-γ-半醛脱氢酶),同时设计引物...
The pto gene, responsible for resistance to Pseudomonas syringae pv. tomato, was transferred to toma...
The pto gene, responsible for resistance to Pseudomonas syringae pv. tomato, was transferred to toma...
Candidatus Liberibacter (Ca. L.) spp. infection in citrus and tomatoes causes reduction of productiv...
Low temperature, as a kind of stress factor, often leads to tomato growth stagnation or yield reduct...
The successful transformation of callus derived protoplasts of the tomato genotype MSK93 was recent...
Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (Fol) is one of the most devastating d...
Genetic engineering has been used as an alternative method to classical breeding procedures to enhan...
通过土壤农杆菌(Agrobacterium tumefaciens)的介导,将烟草花叶病毒(TMV)的54103蛋白cDNA转入番茄植物中,得到了转基因植株.这些植株抗卡那霉素,具有胭脂碱合成酶的活性...
[目的]研究转GO基因番茄的抗病机制.[方法]通过测定转GO基因番茄和对照未转基因番茄接种叶霉菌Fulvia fulva后,体内超氧物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、多酚...
将可以诱发植物对病原菌抗性的甜椒hrap基因置于35S启动子的驱动下,经农杆菌介导引入番茄.再生植株经过含卡那霉素的培养基的选择,后经PCR检测和Southern杂交鉴定含有目标基因.Northern...
对转基因抗黄瓜花叶病毒(CMV)蕃茄进行了安全性评价.结果:CMV蕃茄急性毒性半数致死量LD50>10 g/kg,属实际无急性毒性物质.经微核试验、精子畸变试验及Ames试验均未发现有致突变作用...
本研究室先前的數量性狀基因座遺傳定位研究指出，野生番茄L3708 (Solanum pimpinellifolium L.) 除了在第九對染色體具有晚疫病抗病基因Ph-3以外，於第二對染色體上亦具有另...
The pto gene, responsible for resistance to Pseudomonas syringae pv. tomato, was transferred to toma...
Développer des plantes résistantes aux pathogènes nécessite d’identifier des sources de résistance, ...
从枯草芽孢杆菌(Bacillussubtilis)抗脯氨酸结构类似物突变株中克隆得到脯氨酸合成途径中的关键酶基因proB(编码γ-谷氨酰胺激酶)和proA(编码谷氨酰胺-γ-半醛脱氢酶),同时设计引物...
The pto gene, responsible for resistance to Pseudomonas syringae pv. tomato, was transferred to toma...
The pto gene, responsible for resistance to Pseudomonas syringae pv. tomato, was transferred to toma...
Candidatus Liberibacter (Ca. L.) spp. infection in citrus and tomatoes causes reduction of productiv...
Low temperature, as a kind of stress factor, often leads to tomato growth stagnation or yield reduct...
The successful transformation of callus derived protoplasts of the tomato genotype MSK93 was recent...
Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici (Fol) is one of the most devastating d...
Genetic engineering has been used as an alternative method to classical breeding procedures to enhan...
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