TRANSGENIC TOMATOES WITH TOBACCO MOSAIC VIRUS 54103 PROTEIN GENE AND THEIR RESISTANCE TO TMV INFECTION

程英豪
王继伟
王英典
潘乃遂
陈章良

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Publication date

January 1999

DOI

10.3321/j.issn:0476-0301.1999.01.022

Publisher

北京师范大学学报自然科学版

Journal

issn:0476-0301

Abstract

通过土壤农杆菌(Agrobacterium tumefaciens)的介导,将烟草花叶病毒(TMV)的54103蛋白cDNA转入番茄植物中,得到了转基因植株.这些植株抗卡那霉素,具有胭脂碱合成酶的活性.DNA的PCR扩增及Northernblot分析表明,54103蛋白基因已整合到番茄基因组上.攻毒实验表明,未转基因的对照组接种病毒2周后即出现花叶,并且随着时间的推移,花叶症状更加典型.而转基因的植物接种病毒后一直到开花结果都未见发病,即没有花叶出现.国家重点实验室基金中文核心期刊要目总览(PKU)中国科学引文数据库(CSCD)0193-963

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TRANSGENIC TOMATOES WITH TOBACCO MOSAIC VIRUS 54103 PROTEIN GENE AND THEIR RESISTANCE TO TMV INFECTION

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TRANSGENIC TOMATOES WITH TOBACCO MOSAIC VIRUS 54103 PROTEIN GENE AND THEIR RESISTANCE TO TMV INFECTION

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