International audienceWhile cell analysis is conventionally performed on 2D slices, novel micro and nano-CT system opens new perspectives in this area. Here, we show that synchrotron radiation (SR) micro-CT is well suited to analyze the 3D distribution of osteocyte lacunae in bone tissue. Osteocytes are receiving increasing interest in the comprehension of bone diseases. Here, we propose a fast automated method to extract 3D quantitative morphological descriptors on these cells. To this aim, after a fast connected component analysis applied on the segmented image, a moment-based approach and intrinsic volumes are calculated to derive 3D descriptors on each object. The segmentation is refined by eliminating artifacts according to some descri...