<p>Fold changes of gene expression detected by RNA-Seq were plotted against the data of qRT-PCR. The reference line indicates the linear relationship between the results of RNA-Seq and qRT-PCR. Triangles represent lncRNAs and circles represent protein-coding genes. (B) Relative expression levels of lncRNAs (MSTRG.12377.1, MSTRG.26470.1, MSTRG.28882.1, NONDRET001628.2, MSTRG.2629.2, MSTRG.30168.2, MSTRG.2788.1 and NONDRET001625.2) and their targets genes. (C) Dynamic changes of selected lncRNAs and <i>tp53</i> mRNA during cold acclimation and subsequent recovery process. Data are presented as means ± SD of three independent replicates. ACTB was used as the reference gene. *: p < 0.05, **: p < 0.01, ***: p < 0.001.</p
<p>(A) Dynamic changes in expression profiles of protein-coding genes and lncRNAs. The top panel sho...
<p>(a) Comparisons of changes in miRNAs targets involved in the KEGG pathway. (b) Fold changes of ge...
Long noncoding RNAs (lncRNA) play important roles in gene expression regulation in diverse biologica...
A. The qRT-PCR and RNA-seq results of chronic low-temperature acclimation group. B. The qRT-PCR and ...
<p>(A) Workflow for identification of lncRNAs. The value in parentheses shows the number of transcri...
Data were analyzed by the 2-ΔΔCt method using β-actin as a reference gene. Each column represents th...
<p>(A) A heatmap was generated from the fold change values in the RNA-Seq data, and was used to visu...
<p>The log<sub>2</sub>(fold change) of each lncRNA (left) or its neighboring protein-coding gene (ri...
A. Blue nodes represent upregulated mRNAs; red nodes represent upregulated lncRNAs, and green nodes ...
<p>(A-B) The Volcano plots of differentially expressed lncRNAs and protein-coding genes between cont...
<p>Twenty genes were chosen for RT-qPCR validation. The white and black bars represent the relative ...
<p>(A) Gene expression data for RNA-seq analysis. (B) The RT-qPCR analysis of gene expression data. ...
<p>The letter “A” and “B” in the RNA-Seq and qRT-PCR mean the comparison of expression profiles of s...
<p>Differential expression of twenty genes was analyzed by RT-qPCR and calculated log<sub>2</sub> fo...
<p>Results of qRT-PCR and RNA-seq on genes encoding cold-resistance proteins.</p
<p>(A) Dynamic changes in expression profiles of protein-coding genes and lncRNAs. The top panel sho...
<p>(a) Comparisons of changes in miRNAs targets involved in the KEGG pathway. (b) Fold changes of ge...
Long noncoding RNAs (lncRNA) play important roles in gene expression regulation in diverse biologica...
A. The qRT-PCR and RNA-seq results of chronic low-temperature acclimation group. B. The qRT-PCR and ...
<p>(A) Workflow for identification of lncRNAs. The value in parentheses shows the number of transcri...
Data were analyzed by the 2-ΔΔCt method using β-actin as a reference gene. Each column represents th...
<p>(A) A heatmap was generated from the fold change values in the RNA-Seq data, and was used to visu...
<p>The log<sub>2</sub>(fold change) of each lncRNA (left) or its neighboring protein-coding gene (ri...
A. Blue nodes represent upregulated mRNAs; red nodes represent upregulated lncRNAs, and green nodes ...
<p>(A-B) The Volcano plots of differentially expressed lncRNAs and protein-coding genes between cont...
<p>Twenty genes were chosen for RT-qPCR validation. The white and black bars represent the relative ...
<p>(A) Gene expression data for RNA-seq analysis. (B) The RT-qPCR analysis of gene expression data. ...
<p>The letter “A” and “B” in the RNA-Seq and qRT-PCR mean the comparison of expression profiles of s...
<p>Differential expression of twenty genes was analyzed by RT-qPCR and calculated log<sub>2</sub> fo...
<p>Results of qRT-PCR and RNA-seq on genes encoding cold-resistance proteins.</p
<p>(A) Dynamic changes in expression profiles of protein-coding genes and lncRNAs. The top panel sho...
<p>(a) Comparisons of changes in miRNAs targets involved in the KEGG pathway. (b) Fold changes of ge...
Long noncoding RNAs (lncRNA) play important roles in gene expression regulation in diverse biologica...