Additional file 22: of Tracing the evolution of the heterotrimeric G protein α subunit in Metazoa

Figure S12. DNA- and RNA-binding protein motifs overlapping with the extended conserved region around exon 3 in GNAS of 33 Placentalia. Exon 3 is located at positions 0–46 on the x-axis. A) Local enrichment of known DNA-binding protein (DBP) motifs in comparison to a uniform motif distribution. 30 motifs are enriched in the reported region with a E-value < 0.0001 in all investigated Placentalia; only a subset of these is shown for clarity: Gfi1 (light blue), Hltf (dark blue), EGR1 (pink), MZF1_5–13 (light green), En1 (red), E2F4 (orange), Hoxc9 (dark green). B) Local enrichment of known RNA-binding protein (RBP) motifs in comparison to a uniform motif distribution. Nine motifs are enriched in the reported region with an E-value < 0.0001 in all investigated Placentalia. TARDBP (light blue), DAZAP1 (dark blue), PPRC1 (pink), SRSF9 (light green), SRSF10 (red), CNOT4 (orange), PCBP1 (dark green), KHDRBS1 (black), RBM38 (purple). Note that the SRSF9 binding site is located within the exon and does not overlap with either splice site. C) Local enrichment of RBP sites predicted by Pollard et al. [112]. The respective motifs do not occur in all investigated Placentalia as indicated by a lower probability. SRSF2 (dark blue), SRSF1 (light blue), HNRNPA1 (pink). The 3′ ‘AG’ SS is located at position 0 along the x-axis. The y-axis indicates the probability of a DBP/RBP motif being located centrally at this position. The Figure was created with Centrimo [38]. (PNG 598 kb