Additional file 18: of Tracing the evolution of the heterotrimeric G protein α subunit in Metazoa

Figure S8. DNA- and RNA-binding protein motifs overlapping with the 5′ non-canonical splice site of intron6 in GNAI1. A) Local enrichment of known DNA-binding protein (DBP) motifs in comparison to a uniform motif distribution are shown for Sarcopterygii with ‘GC’ SS (positive set) versus lobe-finned fish and spotted gar with ‘GT’ splice site (SS) (control, adjusted p-value < 0.05). The shown motifs are either present in all species of the positive set and in none of the controls (PRDM1_full, FXR1) or follow this rule with at most one exception. Mafk_secondary UP0004_2 (red), NFIX_full_3 (dark blue), PRDM1_full (green), STAT2:STAT1 (pink, behind green). B) Local enrichment of known RNA-binding protein (RBP) motifs in comparison to a uniform motif distribution. FXR1 (lime green). The SS is located at position 45 along the x-axis. Sequence positions < 45 correspond to exon6, while positions > 45 correspond to intron6. The y-axis indicates the probability of a DBP/RBP motif present centrally at the indicated position for the positive set (solid line) and the control set (dotted line). None of the motifs occurs surprisingly more often at a specific position in the positive set than in the control set (Fisher’s exact test, adjusted p-value < 0.05). The Figure was created with Centrimo [36]. (PNG 287 kb