Receptor protein tyrosine kinase phylogeny

Best of 50 ML trees as inferred by RAxML under the LG+ Γ model (see STAR methods). Purple, orange, blue, and brown texts indicate C. owczarzaki, Pigoraptor (strains Opistho-1 and Opistho-2), S. multiformis (strain Colp-12) and H. sapiens, respectively. The query sequence is indicated in red and with a grey box. An asterisk next to the Opistho-1/-2 and Colp-12 transcript(s) indicates the presence of a transmembrane domain plus a catalytic tyrosine kinase domain. The presence of Fibronectin-3 (FN3) and signal peptides indicating N-terminal complete proteins (SP) or of catalytic tyrosine kinase domains only (TyrKc only) is also indicated. Selected clades are collapsed to increase clarity; annotated H. sapiens homologs in all clades are indicated. Node supports are nonparametric ML bootstrap values obtained from 1000 ML replicates using the LG+ Γ model implemented in RAxML, numbers at nodes represent bootstrap supports of greater than 50%. The scale bar represents the estimated number of amino acid substitutions per site. TIE, tyrosine kinase with immunoglobulin like and EGF like domains; INSR, insulin receptor; INSRR, insulin receptor related receptor; IGF1R, insulin like growth factor 1 receptor; ERBB3, erb-b2 receptor tyrosine kinase 3; EPHB1, EPH receptor B1