Effects of blebbistatin on neuritogenesis of neurons on gel substrates.

<p>All neurons were exposed to 100 mM BS or DMSO for 20 hours. (a) Neurons in stage 1 as a percentage of all the neurons on the gel substrates with/without BS treatment. (n > 300 cells per group, *p<0.001 by one-way ANOVA with Bonferroni post hoc test) (b, c) Fluorescent images of representative neurons on soft substrates (b) and on stiff substrates (c). (d) A magnified image of the boxed area in (c). All scale bars except for (d) are 10 mm.