Increased numbers of cells positive for NeuroD1 and NeuN in nodules of MB allograft treated with AAV-<i>Tis21</i> viral particles.

<p>A) Representative images of IHC staining for the differentiation marker NeuroD1 (red) and for the nuclei dye Hoechst (blue). Scale bars: 50 μm. B) Quantification of the percentage ratio of cells positive for NeuroD1 in nodules of MB allograft treated with AAV-<i>Tis21</i> (orange) and AAV-CBA (blue), to the total number of cells (Hoechst-positive). **** p < 0.0001, Mann-Whitney U test. Mice analyzed: n = 7 for AAV-CBA, n = 7 for AAV-<i>Tis21</i>; fields analyzed: n = 201 for AAV-CBA, n = 207 for AAV-<i>Tis21</i>. C) Representative images of IHC staining for the differentiation marker NeuN (green). In blue the nuclei. Scale bars: 50 μm. D) Quantification of the percentage of NeuN positive cells to the total number of cells (Hoechst-positive). Orange bar (AAV-<i>Tis21</i>), blue bar (AAV-CBA). **** p < 0.0001, Mann-Whitney U test. Mice analyzed: n = 2 for AAV-CBA, n = 3 for AAV-<i>Tis21</i>; fields analyzed: n = 31 for AAV-CBA, n = 41 for AAV-<i>Tis21</i>.