Detection of EMT markers in the non-invasive and invasive tumor in TNBC-MIND model.

<p><b>(A)</b> Representative photographs of Cytokeratin 19 and β-catenin immunostaining serial sections of mammary glands of FVB/N mice injected with Mvt-1 cells for various times (n = 5). Scale bar, 25–200 μm. <b>(B)</b> Representative photographs of Twist and Vimentin immunostaining serial sections of mammary glands of female FVB/N mice injected with Mvt-1 cells for various times (n = 5). Scale bar, 20–200 μm. <b>(C)</b> Representative photographs of Cytokeratin 19 and β-catenin immunostaining sections of lungs illustrating no expression of epithelial markers in metastatic tumors generated by Mvt-1 cells in lungs (n = 5). Scale bar, 50–200 μm. (<b>D)</b> Representative photographs of Twist and Vimentin immunostaining sections of lungs illustrating elevated levels of mesenchymal markers in metastatic tumors generated by Mvt-1 cells in the lungs (n = 5). Scale bar, 100–200 μm.