<i>Bm</i>L3AAMϕ are necessary to sustain a larvicidal tissue eosinophilia.

<p>Cytospins of FACS-sorted BALB/c WT peritoneal SigLecF+ eosinophils <b>(A)</b> or <i>Bm</i>L3AAMϕ <b>(B)</b> +14 days post-infection with 50 <i>Bm</i>L3. Survival analysis of <i>Bm</i>L3 <b>(C)</b> throughout 7-days culture with normal mouse serum (NS) or co-cultured with 10⁶ FACS-sorted eosinophils (Eo), 10⁶ <i>Bm</i>L3AAMϕ or combinations of Eo+<i>Bm</i>L3AAMϕ, (cells sourced as for A,B). Data is pooled from two individual experiments evaluating motility of 10 <i>Bm</i>L3 per condition. Significance of Kaplein-Meir survival analysis <i>vs</i> NS serum control is indicated per condition. Eosinophilia <b>(D,E)</b> +6dpi with 50 <i>Bm</i>L3 ip in BALB/c WT mice pre-treated with clodronate liposomes (CL) ip +/- adoptive transfer of 0.75x10⁶ <i>Bm</i>L3AAMϕ ip (cells sourced as for B). Time course of peritoneal eosinophilia in BALB/c SCID mice at indicated time points post infection with 50 <i>Bm</i>L3 ip <b>(F)</b>. Peritoneal eosinophilia <b>(G,H)</b> or recovery of <i>B</i>. <i>malayi</i> larvae <b>(I)</b> at +14 days post-infection with 50 <i>Bm</i>L3 in BALB/c SCID and E) and pre-treatment with either rat IgG or rat αCCR3 antibody. Data from individual mice with median and interquartile range are plotted. Significant differences between groups assessed by Kruskal-Wallis + Dunn’s tests. Data is from an individual experiment or pooled from 2–3 individual experiments per time-point using groups of 4–6 mice per group / time-point.