Exogenous IL-4 bolsters CCR3-dependent eosinophilia and the eosinophilic larvicidal response in SCID mice via <i>Bm</i>L3AAMϕ development.

<p>Schematic of experimental approach <b>(A).</b> Flow cytometric assessments of F4/80 Mϕ or SigLecF eosinophil proportions <b>(B),</b> total peritoneal cell number <b>(C),</b> eosinophil number <b>(D)</b> at +6dpi or larval parasite recoveries <b>(E)</b> at +14dpi in BALB/c SCID mice pre-treated ip with either rat IgG, clodronate liposomes (CL) or rat αCCR3 ip, prior to infection with 50 <i>Bm</i>L3 and/or up to three doses of rat IgG (25μg), IL-4c (1μg rIL-4 complexed to 5μg rat anti-IL-4) delivered 0d, +2d, +/- +7d with or without daily oral dosing with CCR3 inhibitor. Data from individual mice with median and interquartile range are plotted. Significant differences between test groups and appropriate rat IgG treated controls assessed by Kruskal-Wallis + Dunn’s tests. Data is representative of two individual experiments <b>(B,C)</b> or pooled from 2–3 individual experiments using groups of 3–6 mice per group / time-point.