Microbial Biogeography Study - Meyer et al. 2018 ISME-J

The V3 and V4 region of the 16S rRNA gene of the DNA and cDNA amplified using the primers 319F and 806R. Data are demultiplexed, quality-filtered, trimmed fasta files with primers removed. <div><br></div><div>OTU tables are from 2 community inference methods: DNA- based and RNA (cDNA)- based. OTU tables also differ by similarity threshold, i.e. 95, 97, 99, and 100% sequence similarity. None of these tables have been rarefied or normalized, thus differences in sequencing depth (i.e. sampling extent) across samples will vary. Naming convention is as follows: 'otu_table_rabi_uparse_{OTU sequence similarity threshold}_{community inference method}.txt'. Only OTU tables at 97% cutoff have taxonomic assignments. </div><div><br></div><div>'Rabi_geodist_matrix.txt' is a matrix of pairwise geographic distance (in meters) between samples. </div><div><br></div><div>'soil_chemical_data_pared.txt' is a table with soil chemical attributes as columns and samples as rows. </div><div><br></div><div>'Sampling_effort_DD.R' is an R function that performs a single rarefaction of a community matrix, calculates the slope </div><div>of the distance-decay relationship of that community, then repeats iteratively.</div><div><br></div><div>Please cite: Meyer et al. 2018 ISME-J, DOI: 10.1038/s41396-018-0103-3