Structure and diversity of microbial communities are an important research topic in biology, since microbes play essential roles in the ecology of various environments. Different DNA isolation protocols can lead to data bias and can affect results of next-generation sequencing. To evaluate the impact of protocols for DNA isolation from soil samples and also the influence of individual handling of samples, we compared results obtained by two researchers (R and T) using two different DNA extraction kits: (1) MO BIO PowerSoil((R)) DNA Isolation kit (MO_R and MO_T) and (2) NucleoSpin((R)) Soil kit (MN_R and MN_T). Samples were collected from six different sites on Okinawa Island, Japan. For all sites, differences in the results of microbial com...
Many studies have evaluated the effects of biochar application on soil structure and plant growth. H...
Soil DNA extraction has become a critical step in describing microbial biodiversity. Historically, a...
All molecular analyses of soil bacterial diversity are based on the extraction of a representative f...
Structure and diversity of microbial communities are an important research topic in biology, since m...
During the last two decades, novel molecular and robust methods were developed that were well-suite...
Currently, characterization of soil microbial communities relies heavily on the use of molecular app...
International audienceThree soil DNA extraction procedures (homemade protocols and commercial kit) v...
EA SPE EcolDur GenoSol CT3EASPEEcolDurGenoSolCT3For the last two decades, soil microbial diversity s...
Despite recent technological advances in molecular ecology, DNA extraction from soil remains a cruci...
Despite recent technological advances in molecular ecology, DNA extraction from soil remains a cruci...
International audienceDespite recent technological advances in molecular ecology, DNA extraction fro...
<div><p>Soil DNA extraction has become a critical step in describing microbial biodiversity. Histori...
Soil DNA extraction has become a critical step in describing microbial biodiversity. Historically, a...
Although Next-Generation Sequencing techniques have increased our access to the soil microbiome, eac...
Soil DNA extraction has become a critical step in describing microbial biodiversity. Historically, a...
Many studies have evaluated the effects of biochar application on soil structure and plant growth. H...
Soil DNA extraction has become a critical step in describing microbial biodiversity. Historically, a...
All molecular analyses of soil bacterial diversity are based on the extraction of a representative f...
Structure and diversity of microbial communities are an important research topic in biology, since m...
During the last two decades, novel molecular and robust methods were developed that were well-suite...
Currently, characterization of soil microbial communities relies heavily on the use of molecular app...
International audienceThree soil DNA extraction procedures (homemade protocols and commercial kit) v...
EA SPE EcolDur GenoSol CT3EASPEEcolDurGenoSolCT3For the last two decades, soil microbial diversity s...
Despite recent technological advances in molecular ecology, DNA extraction from soil remains a cruci...
Despite recent technological advances in molecular ecology, DNA extraction from soil remains a cruci...
International audienceDespite recent technological advances in molecular ecology, DNA extraction fro...
<div><p>Soil DNA extraction has become a critical step in describing microbial biodiversity. Histori...
Soil DNA extraction has become a critical step in describing microbial biodiversity. Historically, a...
Although Next-Generation Sequencing techniques have increased our access to the soil microbiome, eac...
Soil DNA extraction has become a critical step in describing microbial biodiversity. Historically, a...
Many studies have evaluated the effects of biochar application on soil structure and plant growth. H...
Soil DNA extraction has become a critical step in describing microbial biodiversity. Historically, a...
All molecular analyses of soil bacterial diversity are based on the extraction of a representative f...