Decreased DG GCL volume of postnatal CRAF ko mice.

<p>(A) Immuno-histological analysis of Nissl stained sagittal brain sections of CRAF ct and CRAF ko hippocampus at P10. (Upper panel) Representative brain section of CRAF ko (right) reveals no general morphological alterations of the hippocampus compared to CRAF ct (left). Scale bar = 100μm. (Lower panel) Higher magnification of CRAF ct (left panel) and CRAF ko (right panel) dentate gyrus granule cell layer (GCL). CRAF ko slices show clusters of darkly stained Nissl cells in the subgranular zone of the dentate gyrus GCL (white arrowheads). Scale bar = 50μm. (B) Quantitative analysis of Nissl-stained dentate gyrus (DG) GCL of CRAF ct (dark bar) and CRAF ko (white bar) at P10 (n = 8). Data are mean ± s.e.m.; no significant differences were detected. (C) (Upper panel) Representative sagittal sections of P30 hippocampus of CRAF ct (left panel) and CRAF ko (right panel) stained with Nissl. CRAF-deficient mice (right panel) display no alterations in the general morphology of the hippocampus. Scale bar = 100μm. (Lower panel) Higher magnification of CRAF ct (left panel) and CRAF ko (right panel) dentate gyrus granule cell layer (GCL). CRAF ko slices show clusters of darkly stained, chromophilic cells in the subgranular zone and hilus of the dentate gyrus GCL (white arrowheads). Scale bar = 50μm. (D) Volume of Nissl-stained dentate gyrus (DG) GCL of CRAF ct (dark bar) and CRAF ko (white bar) at P30 (n = 30). Data are mean ± s.e.m.; significant differences are shown in p-value p< 0.0001.