The BBSome and ARL6 do not localize within cilia in <i>BBS1</i>-KO cells.

<p>(A–R) Control RPE1 cells (A, D, G, J, M, and P) or the <i>BBS1</i>-KO cell lines #B1-1-23 (B, E, H, K, N, and Q) or #B1-2-21 (C, F, I, L, O, and R) were serum-starved for 24 h and triple immunostained for ARL13B (A–C), ARL6 (D–F), BBS5 (G–I), BBS9 (J–L), IFT88 (M–O), or IFT140 (P–R), and Ac-α-tubulin (A′–R′) and γ-tubulin (A′′–R′′). Insets show enlarged images of the boxed regions. Scale bars, 10 μm. (S)–(U), Control cells and <i>BBS1</i>-KO cells with ciliary localization of ARL6 (S), BBS5 (T), and BBS9 (U) were counted, and the percentages are expressed as stacked bar graphs. Values are means ± SD of three independent experiments. In each set of experiments, 82–152 (S), 109–185 (T), and 95–175 (U) ciliated cells were analyzed and the total numbers of ciliated cells analyzed (<i>n</i>) are shown.