AZI1 knockdown increases ciliary localization of BBS4.

<p><b>A</b>) RPE-1 cells expressing GFP-BBS4 were depleted of AZI1, and the number of cells with ciliary GFP (BBS4) was counted. BBS4 is stained with GFP (green), Acetylated α-tubulin was used to detect cilia (red). <b>B</b>) Graph showing significant increases in ciliary localization of BBS4 upon AZI1 knockdown. <b>C</b>) Depletion of AZI1 in BBS3 and BBS5 depleted cells restores ciliary BBSome localization. RPE-1 cells were transfected with siRNA as indicated, and BBS9 (red) localization was analyzed. Cilia (green) in the insets of figures <b>A</b> and <b>C</b> are slightly shifted to show ciliary localization of BBS proteins. <b>D</b>) Cilia containing BBS9 at different conditions were counted and presented graphically. All data are presented in mean +/− SEM. Significance is calculated using the Student's t-test for C and E, and one way ANOVA for A. P<0.05 is considered significant for each analysis.