Bioactive formulations prepared from fruiting bodies and submerged culture mycelia of the Brazilian edible mushroom Pleurotus ostreatoroseus Singer

Pleurotus ostreatoroseus is a Brazilian edible mushroom whose chemical characterization and bioactivity still remain underexplored. In this study, the hydrophilic and lipophilic compounds as well as the antioxidant, anti-inflammatory and antimicrobial activities of formulations (ethanol extracts) prepared with its fruiting bodies and submerged culture mycelia were compared. The bioactive formulations contain at least five free sugars, four organic acids, four phenolic compounds and two tocopherols. The fruiting body-based formulation revealed higher reducing power, DPPH scavenging activity, β-carotene bleaching inhibition and lipid peroxidation inhibition in brain homogenates than the mycelium-based preparation, as well as higher anti-inflammatory and antimicrobial activities. The absence of hepatotoxicity was confirmed in porcine liver primary cells. These functional responses can be related to the levels of bioactive components including phenolic acids, organic acids and tocopherols.The authors are grateful to Foundation for Science and Technology (FTC, Portugal) for financial support to CIMO (Pest-OE/AGR/UI0690/2014), L. Barros research contract (“Compromisso para a Ciência”) and R.C. Calhelha grant (SFRH/BPD/68344/2010), and to the Serbian Ministry of Education, Science and Technological Development for the Grant No 173032. R.C.G. Correa thanks CAPES Foundation, Ministry of Education of Brazil (CAPES fellow, process number BEX 3974/14-6). R.M. Peralta and A. Bracht are Research Fellows of CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico). The authors also thank to A. Fernandes for all the support in some of the laboratorial work