Desarrollo y validación de un método analítico para la determinación de ciprofloxacina en suero por cromatografía liquida de alta precisión con detección ultravioleta./ development and validation of an analytical method for the determination of ciproflox

Objetivo: Desarrollar un método rápido y sencillo para la determinación de Ciprofloxacina en muestras de suero para ser utilizado en estudios farmacocinéticos. La Ciprofloxacina es un antibiótico que pertenece al grupo de las fluoroquinolonas, comúnmente usado en Colombia con un amplio espectro antibacterial. Diferentes métodos utilizando Cromatografía Liquida de Alta precisión (HPLC) han sido reportados para el análisis de Ciprofloxacina en fluidos biológicos. Materiales y métodos: Se empleó el método Cromatografía Liquida de Alta Precisión (HPLC) con detección ultravioleta a 280 nm, fase móvil isocrática, Dexametasona como estándar interno y un rango de cuantificación de 0,1 a 10 μg/ml. Resultados: Los tiempos de retención fueron 4,93 min y 15,71 minutos para Ciprofloxacina y Dexametasona respectivamente, la precisión y exactitud fueron superiores al 95% y el tiempo total de elución fué de 20 minutos por muestra. Conclusiones: Se desarrolló y validó un método analítico para la determinación de Ciprofloxacina en suero, lo suficientemente sensible, exacto, y reproductible para ser usado en estudios farmacocinéticos. Objective: To develop a rapid and simple method for Ciprofloxacin determination insamples of whey to be used in pharmacokinetic studies. Ciprofloxacin is an antibioticthat belongs to the group of the fluoroquinolone, commonly used in Colombia with anextensive antibacterial spectrum. Several High-Performance Liquid Chromatography(HPLC) methods have been reported for the analysis of ciprofloxacin in biologicalfluids. Materials and methods: the method used was High-Performance LiquidChromatography (HPLC) with ultraviolet detection to 280 nm, mobile phase isocratic,Dexamethasone as internal standard and a range of quantification from 0,1 to 10 μg/ml. Results: The times of retention were 4,93 min and 15,71 min for Ciprofloxacin andDexamethasone respectively, precision and accuracy were More than 95 % and a andelution time per sample was 20 minutes. Conclusions: Was developed and validateda sensitive and accurate analytical methods for Ciprofloxacin determination in serum,It is the sufficiently reproducible to be used in pharmacokinetic studiesTo develop a rapid and simple method for Ciprofloxacin determination in samples of whey to be used in pharmacokinetic studies. Ciprofloxacin is an antibiotic that belongs to the group of the fluoroquinolone, commonly used in Colombia with anextensive antibacterial spectrum. Several High-Performance Liquid Chromatography (HPLC) methods have been reported for the analysis of ciprofloxacin in biological fluids. Materials and methods: the method used was High-Performance LiquidChromatography (HPLC) with ultraviolet detection to 280 nm, mobile phase isocratic, Dexamethasone as internal standard and a range of quantification from 0,1 to 10 μg/ ml. Results: The times of retention were 4,93 min and 15,71 min for Ciprofloxacin and Dexamethasone respectively, precision and accuracy were More than 95 % and a and elution time per sample was 20 minutes. Conclusions: Was developed and validateda sensitive and accurate analytical methods for Ciprofloxacin determination in serum, It is the sufficiently reproducible to be used in pharmacokinetic studie