A VALIDATED SECOND DERIVATIVE VISIBLE SPECTROPHOTOMETRIC PROCEDURE FOR THE ASSAY OF CIPROFLOXACIN IN PHARMACEUTICAL DOSAGE FORMS USING FE (III) IN BUFFER MEDIA

Abstract: Fe(III) and ciprofloxacin form a yellow colored complex at pH 2.5. The second derivative spectrum of the complex shows a maximum at 510 nm and a valley at 445 nm and zero point at 478 nm. The derivative amplitude of the complex at 445 nm is proportional to the amount of ciprofloxacin. A plot between the amount of ciprofloxacin and the derivative amplitude at 445 nm is linear which obeys the equation A 445 = 0.0086 C + 0.0003. The linear plot shows that Beer's law is obeyed in the range 1.0-50.0 µg/ml of ciprofloxacin. The standard deviation of the method for ten determinations of 10 µg/ml of ciprofloxacin is 0.0046. The correlation coefficient () is 0.9999. The effect of excipients that are generally associated with ciprofloxacin in pharmaceutical dosage forms is investigated. The proposed visible second derivative spectrophotomeric method was validated as per ICH specifications. The validation parameters such as, linearity, accuracy, precision, LOD, LOQ and robustness were investigated. The method is simple, rapid, precise, selective and accurate. The present method is applied for the determination of ciprofloxacin in pharmaceutical dosage forms