DNA gel retardation assays and AFM images of HP-NAP–DNA complexes at different pH values

<p><b>Copyright information:</b></p><p>Taken from "The neutrophil-activating Dps protein of , HP-NAP, adopts a mechanism different from Dps to bind and condense DNA"</p><p></p><p>Nucleic Acids Research 2007;35(7):2247-2256.</p><p>Published online 19 Mar 2007</p><p>PMCID:PMC1874666.</p><p>© 2007 The Author(s)</p> () pH 6.5; () pH 7.0; () pH 7.5; () pH 8.0; () pH 8.5. In all gels (left panels), 1 refers to linearized plasmid DNA alone (20 nM) and 2 to DNA with HP-NAP at a DNA:protein molar ratio of 1:25. In the AFM images (right panels), the large white globular features in A and B represent HP-NAP–DNA aggregates involving one or few DNA molecules and many HP-NAP dodecamers, those in C, D and E represent single HP-NAP dodecamers. Agarose gels were stained with ethidium bromide. In all AFM images the scan size is 2 μm