Analysis of unspliced (FL) and singly spliced mRNAs in wt and mutant HIV-1 particles

<p><b>Copyright information:</b></p><p>Taken from "HIV controls the selective packaging of genomic, spliced viral and cellular RNAs into virions through different mechanisms"</p><p></p><p>Nucleic Acids Research 2007;35(8):2695-2704.</p><p>Published online 10 Apr 2007</p><p>PMCID:PMC1885669.</p><p>© 2007 The Author(s)</p> Singly spliced () and FL () RNAs were detected in RNA samples extracted from wt (lanes 1 and 4), ΔSL1SL3 (lanes 2 and 5) and mock (lanes 3 and 6) transfected cells and in corresponding viral particles (lanes 4–6) using standard RT-PCR. As a control, amplification was also performed using pNL4.3 plasmid as a template (lane 8). Bands corresponding to vif-2, vpr-3 and env-1 mRNAs are indicated by arrows. In the mutant virus, all amplicons were shortened by 34 nt, due to the SL1 deletion. This result is representative of at least three independent experiments