Sequence Analysis of <i>In Vivo</i>-Expressed HIV-1 Spliced RNAs Reveals the Usage of New and Unusual Splice Sites by Viruses of Different Subtypes - Fig 9

<p><b>Alternative 3’ss usage by (a) <i>rev</i> and (b) <i>env-vpu</i> RNAs.</b> 3’ss separated by one or two nt (A4a/A4e, A4c/A4i, A4d/A4f/A4j) were grouped together. A4e is used by SPX24 and SPX35, A4d by SPX26, A4f by SPX10, A4j by SPX21, and A4i by SPX12. The vertical axis represents proportions of RNAs using each 3’ss relative to all transcripts of each class. Mean proportions of all samples are shown on the right of the columns for individual samples. For comparison, proportions reported in <i>in vitro</i> studies by Purcell and Martin [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158525#pone.0158525.ref006" target="_blank">6</a>] and Ocwieja et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0158525#pone.0158525.ref009" target="_blank">9</a>] are shown.