Interactive effects of resveratrol and TRAIL on cell viability, colony formation and apoptosis in prostate cancer cells

<p><b>Copyright information:</b></p><p>Taken from "Sensitization of TRAIL-resistant LNCaP cells by resveratrol (3, 4', 5 tri-hydroxystilbene): molecular mechanisms and therapeutic potential"</p><p>http://www.jmolecularsignaling.com/content/2/1/7</p><p>Journal of Molecular Signaling 2007;2():7-7.</p><p>Published online 24 Aug 2007</p><p>PMCID:PMC2018690.</p><p></p> (A), Effects of resveratrol and/or TRAIL on cell viability in LNCaP cells. Cells were treated with various doses of resveratrol (0–30 μM) in the presence or absence of TRAIL (50 nM) for 48 h. Cell viability was measured by XTT assay as described in materials and methods. Data represent mean ± SD. * = Significantly different from respective control, P < 0.05. (B), Effects of resveratrol and/or TRAIL on apoptosis in human normal prostate epithelial cells (PrEC). PrEC were treated with resveratrol (20 μM) in the presence or absence of TRAIL (50 nM) for 48 h, and apoptosis was measured by TUNEL assay. (C), Effects of resveratrol and/or TRAIL on colony formation by LNCaP cells. Cells were treated with various doses of resveratrol (0–30 μM) in the presence or absence of TRAIL (50 nM). After three weeks, no of colonies were stained and counted. Data represent mean ± SD. * = Significantly different from respective control, P < 0.05. (D), Effects of different treatment combinations of resveratrol and TRAIL on apoptosis. LNCaP cells were treated with resveratrol (20 μM) in the presence or absence of TRAIL (50 nM). TRAIL was added simultaneously with resveratrol (cotreatment), before or after 24 h of resveratrol treatment. Apoptosis was measured by TUNEL assay. Data represent mean ± SD. * = Significantly different from respective control, P < 0.05