Cerebellar gene expression profiles of mouse models for Rett syndrome reveal novel MeCP2 targets-7

<p><b>Copyright information:</b></p><p>Taken from "Cerebellar gene expression profiles of mouse models for Rett syndrome reveal novel MeCP2 targets"</p><p>http://www.biomedcentral.com/1471-2350/8/36</p><p>BMC Medical Genetics 2007;8():36-36.</p><p>Published online 20 Jun 2007</p><p>PMCID:PMC1931432.</p><p></p>tched: coding sequence. Grey: non-coding parts of transcript. Exons 1 and 2 that are alternatively spliced, and intron 2, are not drawn to scale. Horizontal bar above exon 4 denotes location of the amplicon analyzed by qRT-PCR (in Figure 1B) The (J-allele) mutant has a deletion of exon 3 that encodes the N-terminal half of the MBD. In the (B-allele) mutant, exon 3, the coding portion of exon 4 and part of the 3'UTR are deleted. This larger deletion eliminates ~ 97% of the coding sequence. Using primers within the deleted region, transcript was undetectable in mutant mice with the B-allele, when four wild-type and four mutant samples were compared at 8 wk. This proves the specificity of the primers. In mice with the J-allele, mean expression was higher in the mutants, but this difference was not statistically significant (= 0.11), when six wild-type and seven mutant samples were compared at 8 wk