And were selected for validation of expression changes by qRT-PCR analyses in B-allele mice

<p><b>Copyright information:</b></p><p>Taken from "Cerebellar gene expression profiles of mouse models for Rett syndrome reveal novel MeCP2 targets"</p><p>http://www.biomedcentral.com/1471-2350/8/36</p><p>BMC Medical Genetics 2007;8():36-36.</p><p>Published online 20 Jun 2007</p><p>PMCID:PMC1931432.</p><p></p> Cerebellum microarray expression data (M) are compared with qRT-PCR data from cerebellum (RC), forebrain (RF) and hippocampus (RH). For RC of and , the 8 wk samples from the microarray analysis were used. RC2 data are derived from an independent set of litters at 7.5 to 8 wk. Error bars represent SEM. One asterisk indicates < 0.05, two asterisks indicate < 0.001. expression was markedly increased in the cerebellum of 8 wk B-allele mice on microarray (2-fold, = 0.011, four mutant/wild-type pairs) and qRT-PCR (3.5 fold, < 0.001, four mutant/wild-type pairs) as well as in the forebrain of a different set of mice at 7.5 wk (1.9 fold, < 0.05, three mutant/five wild-type). expression was elevated 1.4-fold on microarrays (< 0.05, five mutant/wild-type sibs) and qRT-PCR (RC) = 0.053, four mutant/wild-type sibs). The RC2 data (five mutant/three wild-type) were more variable and yielded = 0.16. displayed an ~ 1.5 fold increase in expression on microarrays (= 0.03, four mutant/wild-type pairs) and qRT-PCR (= 0.06, five mutant/wild-type pairs) at 2 wk (RC) and was not changed in hippocampus (RH). On microarray, expression was increased at 8 wk, but by qRT-PCR we found highly variable expression in cerebellum (RC and RC2). expression was not significantly increased (= 0.29, eight mutant/eight wild-type) in the forebrain