Comparison of desensitization of currents elicited by GABA applications between α1β2γ2 and α3β2γ2 channels

<p><b>Copyright information:</b></p><p>Taken from "Desensitization and binding properties determine distinct α1β2γ2 and α3β2γ2 GABA receptor-channel kinetic behavior"</p><p></p><p>The European Journal of Neuroscience 2007;25(9):2726-2740.</p><p>Published online 01 May 2007</p><p>PMCID:PMC1950087.</p><p>© The Authors (2007). Journal Compilation © Federation of European Neuroscience Societies and Blackwell Publishing Ltd</p> (A) Normalized average of five responses induced by 3 s applications of saturating GABA (GABA Sat.) to a patch excised from human embryonic kidney cells expressing α1β2γ2 (10 m GABA) and α3β2γ2 (50 m GABA) receptors. Average currents are shown superimposed with a thick line illustrating current from α1β2γ2 receptors and a thin line illustrating that from α3β2γ2 receptors. The initial phase of the GABA currents is shown at an expanded scale in an inset at the bottom right. Traces are normalized to the peak amplitude. (B) Summary of the relative contributions of each component to the peak amplitude (A1–A3 and steady-state) of triple exponential curves used to fit the decay of α1β2γ2 and α3β2γ2 receptor GABA-gated currents to a steady-state value (SS). (C) Each of the three time constants used for the fitting is reported for α1β2γ2 and α3β2γ2 receptors. Each bar represents the mean ± SEM of three patches for α1β2γ2 receptors and seven patches for α3β2γ2 receptors studied per each subunit combination. * < 0.05 indicates a significant difference between subunits