Comparison of deactivation kinetics for currents elicited by brief GABA pulses between α1β2γ2 and α3β2γ2 channels

<p><b>Copyright information:</b></p><p>Taken from "Desensitization and binding properties determine distinct α1β2γ2 and α3β2γ2 GABA receptor-channel kinetic behavior"</p><p></p><p>The European Journal of Neuroscience 2007;25(9):2726-2740.</p><p>Published online 01 May 2007</p><p>PMCID:PMC1950087.</p><p>© The Authors (2007). Journal Compilation © Federation of European Neuroscience Societies and Blackwell Publishing Ltd</p> (A) Normalized average of 10 responses induced by 2 ms applications of saturating GABA (GABA sat.) to a patch excised from human embryonic kidney cells expressing α1β2γ2 (10 m GABA) and α3β2γ2 (50 m GABA) receptors Average currents are shown superimposed with a thick line for α1β2γ2 receptors and a thin line for α3β2γ2 receptors. Inset: the same currents at an expanded time scale. (B–E) Summary of the parameters derived from a triple exponential fitting of decay of α1β2γ2 and α3β2γ2 receptor GABA-gated currents with (in B) the relative contribution of each component to peak amplitude (A–A) and (in C–E) each of the time constants. (F) Weighted averages of deactivation time constants are shown for α1β2γ2- and α3β2γ2-mediated currents. Each bar represents the mean ± SEM of six patches for α1β2γ2 receptors and nine patches for α3β2γ2 receptors studied. Traces are normalized to the same peak amplitude. * < 0.05 indicates a significant difference between subunits