Therapeutic effects of STAT3 decoy oligodeoxynucleotide on human lung cancer in xenograft mice-3

<p><b>Copyright information:</b></p><p>Taken from "Therapeutic effects of STAT3 decoy oligodeoxynucleotide on human lung cancer in xenograft mice"</p><p>http://www.biomedcentral.com/1471-2407/7/149</p><p>BMC Cancer 2007;7():149-149.</p><p>Published online 4 Aug 2007</p><p>PMCID:PMC1988829.</p><p></p> were harvested and the total cellular RNA was isolated using Trizol Reagent, mRNA levels of STAT3-correlated genes such as mcl-1, cyclin D1, bcl-xl, cyclinE, c-myc and survivin were detected using RT-PCR method. The PCR products were electrophoresed and photographed using AlphaEaseFC. After transfected with 25 nmol/L decoy ODN, the whole A549 were harvested and the whole-cell extracts were obtained. And western blotting for bcl-x1 and cyclin D1 were then examined as described in materials and methods. Histogram presented the relative expression level of each gene or protein after normalization to its corresponding internal control. Statistical significance was determined as *< 0.01 and < 0.05 compared to control