Effects of STAT3 decoy oligonucleotide on the proliferation, apoptosis, and migration of HUVECs and HDMECs<i>in vitro</i>.

<p>(A) HUVECs and (B) HDMECs were transfected with the STAT3 decoy oligonucleotide or mutant control oligonucleotide at 0 nM to 1000 nM. Seventy two hours later the cells were assessed for proliferation using MTT assay. Results are shown in percent kill relative to the mutant control oligonucleotide and the experiments were performed in triplicate. (C) HUVECs were transfected with the either STAT3 decoy oligonucleotide (0 nM to 100 nM), the mutant control oligonucleotide (100 nM), vehicle, or cisplatin (100 µM). Twenty four hours later the cells were analyzed for apoptosis using Annexin V staining and flow cytometry. The experiments were performed in triplicate (* p<0.05). (D) HUVECs were transfected with 100 nM of STAT3 decoy oligonucleotide or 100 nM of STAT3 of mutant control oligonucleotide, plated inTranswell8uM pore inserts, and extent of migration was ascertained 24 hrs later. STAT3 decoy oligonucleotide decreased the migration of HUVEC compared with the mutant control oligonucleotide (* p<0.05). All of the experiments were performed in triplicate.