STAT3 decoy inhibits net migration of HUVECs during <i>in vitro</i> tubule formation.

<p>HUVECs were transfected with 100× every ten minutes for the following 24 hours in eight different fields. (A) The decoy was FAM-tagged (green) in order to ensure positive transfection. (B) Representative images from various time points from the course of the experiment.The live cell images were quantified for three distinct parameters: (C) mean distance traveled,(D) mean velocity, and (E) distance from origin. The distance from origin was significantly decreasedby STAT3 decoy oligonucleotide compared to the STAT3 mutant oligonucleotide (** p<0.05). All of the experiments were performed in triplicates.