New insight into the role of the β3 subunit of the GABA-R in development, behavior, body weight regulation, and anesthesia revealed by conditional gene knockout-6

<p><b>Copyright information:</b></p><p>Taken from "New insight into the role of the β3 subunit of the GABA-R in development, behavior, body weight regulation, and anesthesia revealed by conditional gene knockout"</p><p>http://www.biomedcentral.com/1471-2202/8/85</p><p>BMC Neuroscience 2007;8():85-85.</p><p>Published online 10 Oct 2007</p><p>PMCID:PMC2100059.</p><p></p>(yellow boxes) that were used for Southern blot analysis. The targeting construct included a neomycin (neo) cassette that was flanked by two frt sites (blue triangles), two loxP sites (red triangles), a thymidine kinase (TK) cassette, and plasmid vector backbone (broken, wavy line). During vector construction, the BglII restriction sites shown in grey lettering were destroyed. Also shown is a correctly targeted locus (Fneo), a locus following FLPe mediated deletion of the neo cassette (F), and a locus following Cre mediated deletion of exon 3 (f). Abbreviations: BamHI, B; BglII, Bg; EcoRV, RV