Precision and linearity targets for validation of an IFNγ ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides-3

Onder. The same non-responding donor was used for all assays. Unstimulated background was subtracted for each dilution point in CFC and ELISPOT assays. This background was uniformly low (< 0.08% for CFC and < 15 SFC per 2.5 × 10PBMC for ELISPOT) despite the used of allogeneic PBMC for the dilution. Note that the pp65peptide response of this donor (#43) was much lower in ELISPOT compared to the other two assays.<p><b>Copyright information:</b></p><p>Taken from "Precision and linearity targets for validation of an IFNγ ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides"</p><p>http://www.biomedcentral.com/1471-2172/9/9</p><p>BMC Immunology 2008;9():9-9.</p><p>Published online 17 Mar 2008</p><p>PMCID:PMC2275721.</p><p>