Abstract Background Single-cell assays of immune function are increasingly used to monitor T cell responses in immunotherapy clinical trials. Standardization and validation of such assays are therefore important to interpretation of the clinical trial data. Here we assess the levels of intra-assay, inter-assay, and inter-operator precision, as well as linearity, of CD8+ T cell IFNγ-based ELISPOT and cytokine flow cytometry (CFC), as well as tetramer assays. Results Precision was measured in cryopreserved PBMC with a low, medium, or high response level to a CMV pp65 peptide or peptide mixture. Intra-assay precision was assessed using 6 replicates per assay; inter-assay precision was assessed by performing 8 assays on different days; and inte...
With the advent of novel strategies to induce tolerance in autoimmune and autoimmune-like conditions...
The enzyme linked immunospot (ELISpot) assay is a fundamental tool in cellular immunology, providing...
Cytomegalovirus (CMV) infection is one of the most important infectious complications of transplanta...
Ys on different days (inter-assay precision), or three operators on the same day (inter-operator pre...
Onder. The same non-responding donor was used for all assays. Unstimulated background was subtracted...
Nd ELISPOT assays. Circles represent donor 41; triangles, donor 68; and squares, donor 43. Open symb...
Or that donor)×100. This allowed SD to be compared on the same scale. Note that the regression line ...
Enumeration of anti-viral CD8+ T cells to make comparisons between mice, viruses and vaccines is a f...
Enumeration of anti-viral CD8(+) T cells to make comparisons between mice, viruses and vaccines is a...
Major histocompatibility complex (MHC) multimers that identify antigen- specific T cells, coupled wi...
Enumeration of anti-viral CD8+ T cells to make comparisons between mice, viruses and vaccines is a f...
BACKGROUND: The FluoroSpot assay, an advancement of the ELISpot assay, enables simultaneous measurem...
Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following hematopoietic ...
Background: In healthy individuals, Cytomegalovirus (CMV) infection is efficiently controlled by CMV...
Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following hematopoietic ...
With the advent of novel strategies to induce tolerance in autoimmune and autoimmune-like conditions...
The enzyme linked immunospot (ELISpot) assay is a fundamental tool in cellular immunology, providing...
Cytomegalovirus (CMV) infection is one of the most important infectious complications of transplanta...
Ys on different days (inter-assay precision), or three operators on the same day (inter-operator pre...
Onder. The same non-responding donor was used for all assays. Unstimulated background was subtracted...
Nd ELISPOT assays. Circles represent donor 41; triangles, donor 68; and squares, donor 43. Open symb...
Or that donor)×100. This allowed SD to be compared on the same scale. Note that the regression line ...
Enumeration of anti-viral CD8+ T cells to make comparisons between mice, viruses and vaccines is a f...
Enumeration of anti-viral CD8(+) T cells to make comparisons between mice, viruses and vaccines is a...
Major histocompatibility complex (MHC) multimers that identify antigen- specific T cells, coupled wi...
Enumeration of anti-viral CD8+ T cells to make comparisons between mice, viruses and vaccines is a f...
BACKGROUND: The FluoroSpot assay, an advancement of the ELISpot assay, enables simultaneous measurem...
Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following hematopoietic ...
Background: In healthy individuals, Cytomegalovirus (CMV) infection is efficiently controlled by CMV...
Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following hematopoietic ...
With the advent of novel strategies to induce tolerance in autoimmune and autoimmune-like conditions...
The enzyme linked immunospot (ELISpot) assay is a fundamental tool in cellular immunology, providing...
Cytomegalovirus (CMV) infection is one of the most important infectious complications of transplanta...