Bridging the phenotypic gap: Real-time assessment of mitochondrial function and metabolism of the nematode -5

GFP and was indicative of expression levels of the fused protein. It remained fairly constant throughout, except for 15 mM NaNwhere a small decrease was observed. The drop in luminescence was consistent with decreased ATP levels caused by NaN. Synchronised L4 larval stage worms (from 45 h post hatch liquid cultures) were used. Error bars show the SEM. The pooled number of worms was 1200 (± 81) per tested condition. . As a result of not feeding, a nematode treated with 1 mM azide for 30 min does not show red fluorescing microspheres in its pharynx. . Red microspheres accumulate in the pharynx of control nematodes exposed to 0 mM azide. . Luciferase activity (measured by luminescence) in lysates of worms exposed to NaN, expressed as % of control values and normalised to protein content. There were no significant changes in luciferase activity with increasing NaNconcentrations (Regression not significant; P = 0.623466), so that luciferase activity did not influence final luminescence. Error bars show the standard error of the mean (SEM). The pooled number of worms was 4000 (± 169) per tested condition.<p><b>Copyright information:</b></p><p>Taken from "Bridging the phenotypic gap: Real-time assessment of mitochondrial function and metabolism of the nematode "</p><p>http://www.biomedcentral.com/1472-6793/8/7</p><p>BMC Physiology 2008;8():7-7.</p><p>Published online 2 Apr 2008</p><p>PMCID:PMC2364618.</p><p>