High resolution melting analysis for rapid and sensitive and mutation detection in formalin fixed paraffin embedded biopsies-0

Ces in the melting of all the other samples are plotted relative to this baseline. : A difference plot of exon 19 using different amounts of template from one sample. Different amounts of template, ranging from 1 ng to 100 ng, were assessed by the exon 19 assay using a sample with a p.E746_A750 deletion mutation. A near identical heteroduplex type melting pattern is observed from each amount of template tested. The wild-type control sample is shown as a horizontal line (in blue). : A difference plot of exon 19 using different samples with different amounts of template. Each sample contained the same p.E746_A750 in-frame deletion mutation but the samples were at different DNA concentrations and from different individuals. A wild-type control sample is shown as a horizontal line (in blue). All samples show different melting patterns compared to the wild-type.<p><b>Copyright information:</b></p><p>Taken from "High resolution melting analysis for rapid and sensitive and mutation detection in formalin fixed paraffin embedded biopsies"</p><p>http://www.biomedcentral.com/1471-2407/8/142</p><p>BMC Cancer 2008;8():142-142.</p><p>Published online 21 May 2008</p><p>PMCID:PMC2408599.</p><p>