(A) Lamp-1 immunoreactivity in control and TRP-ML–deficient cells is indicated by arrowheads

Control or TRP-ML1 siRNA–treated HeLa cells (5 d) were labeled with antibodies directed against the lysosomal membrane protein lamp-1, as described in Materials and methods. Lysosomes from both control and TRP-ML1 siRNA–treated cells are positive for lamp-1 immunoreactivity. Arrowheads indicate lamp-1–positive labeled compartments. Bars: (left) 2 μm; (middle) 500 nm; (right) 500 nm. (B) Colloidal gold inside storage inclusions in TRP-ML–deficient cells. Control or TRP-ML1 siRNA–treated HeLa cells (5 d) were loaded with 10-nm colloidal gold particles and processed for electron microscopy as described in Materials and methods. A large fraction of the inclusions was gold positive in 5-d siRNA cells, indicating that the inclusions were recently formed. Gold was chased for 16 h. Arrowheads indicate colloidal gold–labeled compartments. Bars: (left) 200 nm; (middle) 2 μm; (right) 500 nm.<p><b>Copyright information:</b></p><p>Taken from "Membrane traffic and turnover in TRP-ML1–deficient cells: a revised model for mucolipidosis type IV pathogenesis"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1477-1490.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413042.</p><p>